Reagent mixing and conveying device and reagent mixing method

ABSTRACT

The present invention provides a reagent mixing device, which comprises a driving device, a transport device and a rotating part, wherein the transport device comprises a conveying mechanism for conveying a reagent kit and a mixing mechanism for mixing a reagent; the conveying mechanism is driven by the driving device to move relative to the mixing mechanism; the rotating part and mixing mechanism are in transmission matching; the conveying mechanism and the mixing mechanism are sleeved with each other to form a bearing structure. The present invention further provides a reagent mixing method. The reagent mixing device is small in size, smart in structure, easy to assemble and low in manufacturing cost. The reagent mixing method provided by the present invention is simple and reliable, high in overall operation reliability, and has very high application values in such analysis and test fields as full-automatic chemiluminescence immunoassay analyzers and biochemical analyzers.

FIELD OF THE INVENTION

The present invention relates to a reagent mixing device and a reagentmixing method, in particular to a reagent mixing and conveying devicefor a chemiluminescence immunoassay analyzer.

BACKGROUND OF THE INVENTION

A full-automatic test analyzer is capable of automatically performing aseries of operation steps including sample addition, reagent addition,reaction, test, test result acquisition and the like. It is now verycommon to determine the content of a component in a sample under test byusing a full-automatic detection analyzer, for example, a full-automaticchemiluminescence immunoassay analyzer which includes a sample cabin, areagent cabin, a reaction cabin and a test cabin. The test process ofthe analyzer generally comprises the following steps: first, putting asample and a reagent into the sample cabin and the reagent cabinrespectively; next, adding the sample and the reagent into a cuvette;then, making the cuvette go through systems of incubation, separation,washing and the like; and finally, feeding the cuvette into a closeddark chamber and completing the test.

Chemiluminescence immunoassay (CLIA) is an analysis technique throughwhich a chemiluminescence testing technique with high sensitivity iscombined with immunoreaction with high specificity to quantitativelydetect various antigens, haptens, antibodies, hormones, enzymes, fattyacids, vitamins, medicines and the like. Chemiluminescence immunoassayneeds multiple reagents, including solid-phase reagents, and a commonsolid-phase reagent is marked magnetic particles. When in use, themagnetic particle reagents are required to be uniform in concentration.However, the magnetic particles are apt to deposit under the effect ofgravity, resulting in nonuniformity. If the magnetic particle reagentsparticipate in relevant reactions after deposition, the stability andreliability of testing results can be severely affected. Therefore, themagnetic particle reagents need to be uniformly mixed before use.

In an existing full-automatic chemiluminescence immunoassay analyzer, astirring mechanism is used to stir and uniformly mix the magneticparticle reagents. However, this method is not only long in mixing timeand poor in effect, but also very liable to cause cross pollution. If ahigh-frequency throughout or oscillation method is used to mix themagnetic particle reagents, the requirement that the magnetic particlereagents in a plurality of reagent kits should be simultaneously mixedcannot be met, and the test speed of the full-automatic analyzer can belowered. In the method that the magnetic particle reagents are mixed ina transmission matching manner, an analysis instrument comprises areagent kit conveying part and a magnetic particle reagent mixing part.By adopting a full-automatic analyzer, a corresponding reagent kit canbe conveyed to a reagent acquisition position of the analyzer by theconveying part according to the demands of test items, and in thereagent kit conveying process, the magnetic particle reagents areuniformly mixed by the mixing part in a transmission matching manner.However, in an existing design, the conveying part and the mixing partbelong to independent operation systems. Due to relatively independentand separated structures, the operation accuracy and reliability of theanalyzer can be degraded, and the assembling complexity and theproduction and manufacturing costs of the analyzer can be increased. Thesize of the analyzer itself can also be increased due to complex anddiverse modules of a mechanical structure, so that the analyzer is verylarge, and a relatively large space is needed in a laboratory.

In the existing full-automatic detection analyzer, the matching betweena reagent kit and a reagent kit bracket generally needs complex matchingstructures such as a hook to keep the reagent kit in place on thereagent kit bracket. Therefore, the manufacture of the reagent kit andthe reagent kit bracket is not only time consuming, but also high incost. The reagent kit and the reagent kit bracket are incompact instructure and large in size. Mechanical noise can be generated fromassembled fittings, so that the test precision of the analyzer can beaffected, and moreover, subsequent repairing difficulties and cost canbe increased.

In the test analysis field, multiple reagents are generally needed toaccomplish the test on one item. When the full-automatic test analyzeris adopted to test the item, multiple reagents are gathered in onereagent kit, and the reagent kit is put into the full-automaticanalyzer. A plurality of reagent bottle storage cavities are formed inthe reagent kit, and different reagents are contained in differentreagent bottles. The reagent bottles filled with the reagents are putinto the reagent kit storage cavities in advance.

When different items are tested, the reagent kit in the analyzer needsto be replaced. Or the reagent kit needs to be replaced if the reagentsin the reagent kit are used up. If a reagent kit without a handle isused, an operator has to grasp the body of the reagent kit to take outthe reagent kit. If reagent kits are closely placed inside the analyzer,that is, the reagent kits are arranged close to one another, then thespace reserved for the operator to hold the reagent kits by fingers issmall, and thus the reagent kits cannot be conveniently taken out. Ifthe reagent kit is additionally provided with a handle, although thereagent kit can be very conveniently taken out by the operator, acertain space of the instrument will be occupied by the handle, and theoverall size of the instrument is increased, so that not only is thematerial cost of the instrument increased, but also a relatively largerspace of the laboratory will be occupied.

According to demands of medical test, a plurality of test items need tobe carried out with one sample sometimes. For example, to analyzewhether a patient has a communicable disease, items such as serialHSV-I, HSV-II, RV, HCMV and TOXO, Chlamydia, Gonorrhea, HIV and Syphilisin series HBsAg, HBsAb, HBeAg, HBeAb, HBcAb, HCV and ToRCH of a sampleof the patient need to be tested. To analyze whether the sample of thepatient has a tumor marker, PSA, Cyfra21-1, AFP, CEA, NSE, CA19-9,CA15-3, CA72-4, CA125, CA 50, ProGRP, Fer, TPS, GPC3 and the like needto be tested. To determine whether a patient under test has drug abuse,items such as MOP, AMP, BAR, COC, MET, THC, BZO, MDMA, MTD, OPI and PCPneed to be tested. Different test reagents are needed for different testitems. Therefore, the reagent cabin of the full-automatic analyzer needsto be capable of storing a sufficient number of reagent kits at one timeto satisfy test requirements. If reagent kit storing positions of thereagent cabin are not adequate, in the same test series, such as test oninfectious disease series, reagent kits of corresponding test items needto be put into the analyzer for test in different batches. First,reagent kits for testing HBsAg, HBsAb, HBeAg, HBeAb, HBcAb, HCV,Chlamydia and Gonorrhea are put into the analyzer, at this time, thereagent kit storing positions of the reagent cabin of the analyzer arecompletely occupied, and then test is started. After the first batch ofitems are tested, the reagent kits used in the first batch of test aretaken out, and then test reagent kits of HSV-I, HSV-II, RV, HCMV, TOXO,Chlamydia, Gonorrhea, HIV and Syphilis of a ToRCH series are put in.Therefore, the analysis test progress is affected.

SUMMARY OF THE INVENTION

To solve the problems in the prior art, the present invention provides areagent mixing and conveying device which comprises a driving device, atransport device and a rotating part, wherein the transport devicecomprises a conveying mechanism for conveying a reagent kit and a mixingmechanism for mixing a reagent; the conveying mechanism is driven by thedriving device to move relative to the mixing mechanism; the rotatingpart and the mixing mechanism are in transmission matching; and theconveying mechanism and the mixing mechanism are sleeved with each otherto form a bearing structure.

Further, the conveying mechanism and the mixing mechanism are of annularstructures; and the conveying mechanism is arranged inside a centralhole of the mixing mechanism.

Further, the driving device comprises a driving motor and a driving end;and the driving end is arranged in a central hole of the conveyingmechanism.

Further, the transmission mode between the driving end and the conveyingmechanism is selected from gear engagement transmission, frictiontransmission and belt pulley transmission; and the transmission modebetween the mixing mechanism and the rotating part is selected from gearengagement transmission and friction transmission.

Further, the conveying mechanism is of an internal gear structure; themixing mechanism is of an external gear structure; the driving end isprovided with a driving rack; the driving rack is engaged with theinternal gear of the conveying mechanism; the rotating part is a gear;and the gear as the rotating part is engaged with the external gear ofthe mixing mechanism.

Further, the conveying mechanism comprises a base and a mounting seat;the base and the mixing mechanism are connected with each other in theform of a bearing; the mounting seat is mounted on the base; and themounting seat comprises a transmission part matched with the drivingdevice.

The present invention further provides a test reagent mixing methodwhich comprises the following steps:

(1) providing a reagent mixing and conveying device;

(2) putting a reagent kit filled with a reagent to be mixed on thereagent mixing and conveying device;

(3) starting the reagent mixing and conveying device to uniformly mixthe reagent;

wherein the reagent mixing and conveying device comprises a drivingdevice, a transport device and a rotating part; the transport devicecomprises a conveying mechanism for conveying a reagent kit and a mixingmechanism for mixing a reagent; the driving device comprises a drivingmotor and a driving end; the driving end is arranged in a central holeof the conveying mechanism; the conveying mechanism is driven by thedriving device to move relative to the mixing mechanism; the rotatingpart and the mixing mechanism are in transmission matching; the rotatingpart is used for stirring reagent to be mixed; and the conveyingmechanism and the mixing mechanism are sleeved with each other to form abearing structure.

Further, the conveying mechanism and the mixing mechanism are of annularstructures; and the conveying mechanism is arranged in a central hole ofthe mixing mechanism.

Further, the conveying mechanism is of an internal gear structure; themixing mechanism is of an external gear structure; the driving end isprovided with a driving rack; the driving rack is engaged with theinternal gear of the conveying mechanism; the rotating part is a gear;and the gear as the rotating part is engaged with the external gear ofthe mixing mechanism.

Further, the conveying mechanism is driven by the driving device to moverelative to the mixing mechanism; a corresponding reagent kit isconveyed to a reagent acquisition position of an analyzer by theconveying mechanism; meanwhile, with the rotation of the conveyingmechanism, the rotating part for uniformly mixing the reagent is intransmission with the mixing mechanism; and a reagent bottle autorotatesinside the reagent kit under the transmission of the mixing mechanismand the rotating part, so that the reagent inside the reagent bottle isuniformly mixed under rotation.

The reagent mixing and conveying device provided by the presentinvention is small in size, smart in structure, simple and reliable,easy to assemble, high in overall operation reliability and low inmanufacturing cost. In addition, the mixing method of the magneticparticles of the present invention is simple and reliable, good inmixing effect, free of cross pollution risk, and moreover, a pluralityof magnetic particle agents can be uniformly mixed simultaneously.

By adopting the test reagent storage device provided by the presentinvention, the space is effectively utilized, and the reagent storageamount is increased. For the reagent kit and the reagent kit bracket,due to the matching between positioning parts and positioning slots, thereagent kit can be more firmly placed inside reagent cabin. Such amatching mechanism can effectively reduce shaking of the reagent kit andlower the noise of the analyzer.

Due to the matching structure of clamping parts of the reagent bottleand the reagent kit, a reagent kit body can also be lifted while thereagent bottle is lifted up, so that the whole reagent kit body is keptin balance, and the risk that the reagent is dumped because ofinclination is avoided.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic diagram of a reagent kit body and reagent bottlesseparated from each other.

FIG. 2 is a schematic diagram of the reagent kit body and the reagentbottles assembled together.

FIG. 3 is a schematic diagram illustrating that reagent kits are putinto a reagent kit bracket.

FIG. 4 is a schematic diagram showing the matching between positioningparts arranged in a staggered manner with positioning slots on thebracket.

FIG. 5 is a schematic diagram showing the matching between positioningparts arranged in a corresponding manner with positioning slots on thebracket.

FIG. 6 is a schematic diagram showing the matching between positioningparts arranged on only one side wall of the reagent kit and positioningslots on the bracket.

FIG. 7 is a top view illustrating that a convex block is put into areagent kit body along a clamping part passage.

FIG. 8 is a top view illustrating that a convex block is rotated to bebelow a cover edge.

FIG. 9 is a partial view illustrating that a convex block is put into areagent kit body along a clamping part passage.

FIG. 10 is a partial view illustrating that a convex block is rotated tobe below a cover edge.

FIG. 11 is a schematic diagram showing the matching between an L-shapedstructure part on a reagent bottle and an inverted concave structureinside a kit body.

FIG. 12 is a schematic diagram showing the matching between a bucklepart on a reagent bottle and a buckle groove inside a kit body.

FIG. 13 is a schematic diagram showing the matching between a reagentstorage device and a reagent mixing and conveying device.

FIG. 14 is a schematic diagram of the reagent mixing and conveyingdevice.

FIG. 15 is a schematic diagram of the reagent mixing and conveyingdevice.

FIG. 16 is a top view of a reagent cabin.

FIG. 17 is a sectional view of the reagent cabin of FIG. 16 in an A-Adirection.

FIG. 18 is a schematic diagram of a reagent cabin with reagent kitstherein.

FIG. 19 is a schematic diagram of a reagent cabin with a refrigerationdevice.

FIG. 20 is a schematic diagram of an internal structure of afull-automatic chemiluminescence immunoassay analyzer.

FIG. 21 is a schematic diagram of a full-automatic chemiluminescenceimmunoassay analyzer.

FIG. 22 is a full-automatic chemiluminescence immunoassay analyzerwithout a sample cabin side baffle plate.

DETAILED DESCRIPTION OF THE EMBODIMENTS

The present invention is further described in detail below inconjunction with accompanying drawings and embodiments, but theprotection scope of the invention is not limited thereto.

A full-automatic chemiluminescence immunoassay analyzer 9000, as shownin FIGS. 20 to 22, includes a sample cabin 9300, a reagent cabin 9400, acuvette storage cabin 9500, an incubation cabin 9600, a washing cabin9700 and a test cabin 9800. To analyze components of a sample undertest, firstly, a sample and a reagent are respectively put into thesample cabin and the reagent cabin; a cuvette is taken out of thecuvette storage cabin 9500 of the full-automatic chemiluminescenceimmunoassay analyzer, and is put into the incubation cabin; next, thesample and the reagent are put into the cuvette according to presetprocedures, and an incubation procedure and a washing procedure areimplemented; finally, the cuvette is fed into the test cabin to completesample component analysis. The sample cabin herein includes an identityinformation reading device; and the reagent cabin includes a reagentmixing and conveying device.

As shown in FIGS. 1 and 2, a reagent kit 1 for storing a test reagentincludes a kit body 2 and a mixing cavity 3; the mixing cavity 3 is usedfor storing the reagent to be mixed uniformly during test; the reagentcan be directly fed into the mixing cavity or fed into a reagent bottlein advance and subsequently put into the mixing cavity 3. If necessary,the reagent kit may further include storage cavities 4, which are usedfor storing reagents with relatively low requirement on uniform mixingduring test, and the reagents can be directly fed into the storagecavities, or are fed into reagent bottles in advance and subsequentlyput into the storage cavities 4.

In an embodiment of FIG. 1, the reagent to be uniformly mixed is fedinto a mixing reagent bottle 5 in advance; the mixing reagent bottle 5is provided with a rotating part 6. The mixing reagent bottle 5 filledwith the reagent is put into the mixing cavity 3 of the reagent kit.With rotation of the rotating part 6, the mixing reagent bottle 5 iscaused to rotate inside the mixing cavity 3, so that the reagent insidethe mixing reagent bottle 5 enters a suspension mixing state due torotation. The rotating part 6 can be a structure built in the mixingreagent bottle 5; that is, as shown in FIG. 1, the rotating part 6 hasalready been mounted on the mixing reagent bottle 5 before the mixingreagent bottle 5 is put into the mixing cavity. The rotating part 6 canalso be a separate member; after the mixing reagent bottle 5 is put intothe mixing cavity 3, the mixing reagent bottle cooperates with therotating part, so that the mixing reagent bottle 5 and the rotating part6 are assembled together. When being used as a separate part, therotating part can be mounted on the reagent kit, or arranged on aninstrument used with the reagent kit. In an embodiment of FIG. 2, afterthe mixing reagent bottle 5 is put into the mixing cavity 3, therotating part 6 is positioned at a notch 31 of the mixing cavity 3.

A positioning hole 104 is formed in the wall of each reagent kit storagecavity and/or the mixing cavity; a positioning part 7 is correspondinglyarranged on the tube wall of a reagent bottle 11. Before the reagentbottle is put into the storage cavity and/or the mixing cavity, thereagent bottle positioning part 7 is firstly aligned to the positioningslot 104, and subsequently, the reagent bottle is fed into a admissioncavity and/or the mixing cavity downwards, thus ensuring that thereagent bottle can be accurately placed inside the admission cavityand/or the mixing cavity. The upper surface of the positioning part 7 isflush with that of the reagent bottle; the depth of the positioning slot104 is identical to the longitudinal depth of the positioning part 7.After the positioning part of the reagent bottle is inserted along thepositioning slot, if the upper surface of the positioning part is flushwith that of the positioning slot, it means that the reagent bottle hasbeen accurately positioned in place.

In another embodiment, the inner wall of each storage cavity also has anelastic piece for tightly clamping a reagent bottle inside the storagecavity.

In the test field, such as chemiluminescence immunoassay, multiplereagents are needed to accomplish the test of one item, includingreagents with solid phases, such as reagents with magnetic particles.Therefore, the reagent kit with the mixing cavity 3 and a plurality ofstorage cavities 4 is adopted, and reagents with magnetic particles areput into the mixing reagent bottles.

During test, a reagent kit corresponding to a test item is put into ananalyzer. In one embodiment, the analyzer includes a reagent kit bracketfor holding the reagent kit, and according to type of test item, acorresponding test reagent kit is put in a corresponding position of thereagent kit bracket, and thus multiple items can be tested by startingthe analyzer once.

The reagent kit bracket 100, as shown in FIG. 3, includes an inner ring101 and partitioning plates 102; the partitioning plates are mounted tothe inner ring; a admission cavity 103 of which the horizontal crosssection is an annular sector is formed between every two partitioningplates 102; and the admission cavity is used for storing a reagent kit1. In one embodiment, the number of the admission cavities 103 isconfigured such that a maximum number of reagent kits can be containedin the reagent kit bracket. Due to this configuration, more test itemscan be accomplished, and reagent kits do not need to be placed twice ina series of tests.

In one embodiment, positioning slots 104 are formed in the upper edge ofeach partitioning plate 102 of the reagent kit. Positioning parts 7 arearranged at locations on the reagent kit as shown in FIG. 2corresponding to the partitioning plate positioning slots 104. Beforethe reagent kit is put into the admission cavity of the bracket, thereagent kit positioning parts are aligned to the positioning slots, andthen, the reagent kit is fed into the admission cavity downwards, thusensuring that the reagent kit can be accurately contained inside theadmission cavity 103, so as to guarantee a liquid acquirer of theanalyzer can be accurately fed into the reagent storage cavity or themixing cavity when the reagent is acquired in the test process, therebypreventing the liquid acquirer from touching the upper cover of thereagent kit to cause damage to the liquid acquirer. In anotherembodiment, to reduce the weight of the analyzer, each admission cavityof the reagent kit bracket is vertically cut through, and the bottom ofthe admission cavity is not provided with a bottom plate for supportingthe reagent kit. Therefore, the positioning parts and the positioningslots are matched with each other to support the reagent kit placedinside the admission cavity 103. The longitudinal depth of thepositioning parts 7 is identical to the depth of the positioning slots104. After the reagent kit is put into the admission cavity, if theupper surface of the positioning part is flush with the upper surface ofthe positioning slot, it means that the reagent kit is arranged inplace, and the operation requirement of the analyzer is met. Inembodiments as shown in FIGS. 1 to 3, the upper surfaces 71 of thepositioning parts 7 are flush with the upper surface 8 of the reagentkit, and the depth of the positioning slots 104 is identical to thelongitudinal depth of the positioning parts 7. After the reagent kitpositioning parts are inserted along the positioning slots, if the uppersurfaces 71 of the positioning parts are flush with those of thepositioning slots, it means that the reagent kit is arranged in place.Due to the design that the upper surfaces 71 of the positioning parts 7are flush with the upper surface 8 of the reagent kit, an operator iscapable of observing whether the reagent kit is arranged in place moreconveniently.

In one embodiment, positioning parts on two side walls of a reagent kitare arranged alternately. Specifically, a positioning part is arrangedon the first side wall of the reagent kit, and a positioning part on thesecond side wall of the reagent kit is not arranged at a positioncorresponding to the positioning part on the first side wall. Further,as shown in FIG. 1, a middle positioning part 72 is arranged on one sidewall of the reagent kit, and another side wall of the reagent kitincludes a left positioning part 73 and a right positioning part 74; theleft positioning part and the right positioning part are respectivelypositioned on two corresponding sides of the middle positioning part. Asshown in FIG. 4, according to a method of the embodiment, threepartitioning plates 102 with an identical arrangement of positioningslots 104 form two admission cavities 103; after being put into theadmission cavities, two reagent kits 1 with an identical arrangement ofpositioning parts tightly lean against two sides of the samepartitioning plate. The middle positioning part 72 of the first reagentkit, and the left positioning part 73 and the right positioning part 74of the second reagent kit are matched with the correspondingpartitioning plate positioning slots alternately. The left positioningpart 73 and the right positioning part 74 of the first reagent kit, andthe middle positioning part 72″ of the third reagent kit are matchedwith the corresponding partitioning plate positioning slots alternately.The middle positioning part 72 of the second reagent kit and the leftpositioning part and the right positioning part of the fourth reagentkit are arranged alternately. Due to the design solution, the number ofpartitioning plates can be reduced, so that the size of the analyzer canbe reduced. In addition, as two side walls of each reagent kit aresupported by the partitioning plates, storage stability of the reagentkit inside the admission cavity is ensured. Compared with the design ofFIG. 5, the embodiment as shown in FIG. 4 illustrates that once tworeagent kits are stored, one partitioning plate of the reagent kitbracket can be reduced, therefore, the space occupied by the reagent kitbracket can be reduced, and a larger space can be available to storemore reagent kits as the number of the partitioning plates of thereagent kit bracket is reduced. The positioning parts 7 on two sidewalls of the reagent kit as shown in FIG. 5 are arranged correspondingto each other, and when two reagent kits are put into the admissioncavities, four partitioning plates 102. Compared with the design of FIG.6, the embodiment of FIG. 4 illustrates that the reagent kits are storedmore stably. As shown in FIG. 6, to hold two reagent kits insidecontaining cavities formed by three partitioning plates, only one sidewall of each reagent kit is provided with the positioning parts 7. Afterthe reagent kits are put into the admission cavities 103, the side edgesof the reagent kits without the positioning parts are not supported bythe partitioning plates 102, so that the placement of the reagent kitsin the admission cavities is very unstable.

The inner ring of the reagent kit bracket may further include reagentkit positioning guiding slots 105, and a reagent kit is provided with aguiding part 9. When the reagent kit is put into the admission cavity,the guiding part 9 is inserted into the guiding slot 105 and movesdownwards along the guiding slot. Due to the matching design of theguiding slot and the guiding part, the reagent kit can be rapidly andaccurately put into the admission cavity. In embodiments as shown inFIGS. 2 and 3, a guiding slot 105 is positioned in the middle betweenevery two partitioning plates; the guiding part 9 is positioned in themiddle of the outer side of a narrow end of the reagent kit. In anotherembodiment, a stop block 10 is further mounted on the guiding part, andthe width of the stop block is greater than that of the opening of theguiding slot. As shown in FIG. 3, after the guiding part 9 is insertedinto the guiding slot, the stop block 10 is positioned on the outer sideof the guiding slot 105, and the position of the reagent kit inside theadmission cavity is further limited.

A reinforcing wall 106 may be further arranged on the inner side of theinner ring of the reagent kit bracket; the reinforcing wall 106 furtherensures the shape of the reagent kit bracket, such as a circular shape.In the operation process of the analyzer, the reagent kit bracket of astable shape can ensure accuracy of reagent sampling. After the reagentkit is put into the admission cavity, the bottom of the stop block 10can also abut against the upper surface of the reinforcing wall 106,thus functioning to further support the reagent kit.

A reagent storing device for the full-automatic in vitro diagnosisanalyzer comprises reagent kits 1 and a reagent kit bracket 100 of thepresent invention, wherein the reagent kit bracket includes admissioncavities 103; the number of the admission cavities 103 is configuredsuch that a maximum number of reagent kits can be contained in thereagent kit bracket; each reagent kit includes a reagent storing cavity4 and/or a mixing cavity 3; the number and the size of the reagentstoring cavity 4 and/or the mixing cavity 3 are configured such that amaximum types of reagents and/or a maximum quantity of reagents can bestored in the reagent kit. The reagent kit as shown in FIG. 1 includesthree circular reagent storage cavities 4 and one mixing cavity 3located at a narrow end of the reagent kit; a storage cavity with alarger diameter is formed in the middle of the reagent kit; two storagecavities of the same diameter are formed in parallel at a wide end ofthe reagent kit; the outer edges of the storage cavities and the mixingcavity are tangent to the inner wall of the reagent kit. In oneembodiment, the entire reagent kit is provided with the storage cavitiessolely.

In one embodiment, the horizontal cross sections of the admissioncavities and the reagent kits are both annular sectors. The combinationof the number of the admission cavities and the number of the reagentstorage cavities enables the reagent stored in the reagent storingdevice to reach the maximum amount of the reagent that can be stored inthe reagent storing device.

In one embodiment, the reagent bottle 11 includes a clamping part, andthe storage cavity 4 includes a blocking part. The blocking part is usedfor blocking the clamping part from leaving the storage cavity. When theclamping part is blocked by the blocking part, a reagent kit body can belifted together with a lifted reagent bottle, so that the whole kit bodyis kept in balance, and the danger that the reagent is dumped because ofinclination is avoided.

In embodiments as shown in FIG. 1 and FIGS. 7 to 10, the reagent kit 1includes a kit body 2 and a storage cavity 4. The storage cavity is usedfor storing a reagent or a reagent bottle containing a reagent. Thereagent bottle 11 includes a clamping part; and the storage cavity 4includes a blocking part and a clamping part passage 12. In oneembodiment, the clamping part is a convex block 13 mounted on the outerwall of the reagent bottle; the blocking part is a cover edge 14 of akit body cover; the cover edge is a part of the kit body cover; and thecover edge 14 is closer to the center of the transverse cross section ofthe storage cavity than the inner wall of the storage cavity 3. Theclamping part passage 12 is positioned at the cover and is adjacent tothe blocking part. The convex block 13 is fed into or leaves the storagecavity through the clamping part passage 12. As shown in FIGS. 7 and 9,when the reagent bottle 11 needs to be put into the kit body 1, theconvex block 13 on the reagent bottle is firstly aligned to the clampingpart passage 12, and subsequently the reagent bottle is put into thestorage cavity downwards. As shown in FIGS. 8 and 10, after reaching apreset position, the reagent bottle is rotated, the convex block 13(namely, the clamping part) on the reagent bottle is rotated to the partbelow the cover edge 14 (namely, the blocking part), and then thereagent bottle cannot be moved out of the kit body. When the reagentbottle is lifted up, the kit body is also lifted up, and then thereagent kit can be put into an analysis instrument or can be taken outof the analysis instrument. When the reagent bottle is rotated to rotatethe convex block to the clamping part passage 12, the convex block 13 isnot positioned below the cover edge 14 any longer, so that the reagentbottle can be moved out of the kit body. In a preferred solution, thetail end of the convex block includes a stop part 131, and the stop partis higher than the lower bottom surface of the cover edge. When theconvex block is rotated to be below the cover edge, the stop part cannotpass through the cover edge, which indicates that the convex block isrotated in place.

In an embodiment as shown in FIG. 11, the clamping part is an L-shapedstructural part 15, and the blocking part is an inverted concavestructural part 16. When the reagent bottle reaches a preset position,the reagent bottle is rotated to rotate the L-shaped structural part 15on the reagent bottle into the inverted concave structural part 16, andthen the reagent bottle cannot be taken out of the kit body. When thereagent bottle is lifted, the kit body can also be lifted up.

In an embodiment as shown in FIG. 12, the clamping part is a bucklepart, which includes an elastic clamping arm 17 and a clamping foot 18,one end of the clamping arm 17 being mounted on the outer wall of thereagent bottle. The blocking part is a buckle groove 19. When thereagent bottle is stored inside the storage cavity, the clamping arm 17is pressed to the outer wall of the reagent bottle and is moveddownwards along a vertical plate 20 of the buckle groove 19 till theclamping foot 18 is buckled inside the buckle groove 19, and thevertical plate 20 of the buckle part prevents the clamping foot frommoving out of the storage cavity. When the reagent bottle needs to bereplaced, the reagent bottle can be taken out of the kit body by onlypressing the clamping arm 17 to the outer wall of the reagent bottleagain to disengage the clamping foot 18 from the buckle groove 19.

The reagent mixing and conveying device includes a transport device anda driving device; the transport device includes a conveying mechanism201 and a mixing mechanism 202; the conveying mechanism 201 and themixing mechanism 202 are sleeved with each other to form a bearingstructure; the conveying mechanism is used for holding reagent kits andconveying the reagent kits to a corresponding position of the analyzer;the mixing mechanism 202 is matched with a rotating part 6 and is usedfor mixing a reagent to be uniformly mixed inside the reagent kits; thedriving device includes a driving end 301 and a power part 302; thedriving device drives the conveying mechanism 201 to move relative tothe mixing mechanism, and enables the rotating part 6 for mixing thereagent to be in transmission with the mixing mechanism 202, so as toachieve transferring and uniform mixing of test reagent.

In embodiments as shown in FIGS. 13 to 17, the conveying mechanism 201and the mixing mechanism 202 are of annular structures; the conveyingmechanism is arranged in the central hole of the mixing mechanism; andthe conveying mechanism 201 and the mixing mechanism 202 are assembledtogether to form a bearing structure. In an embodiment as shown in FIG.15, beads 203 are arranged between the conveying mechanism 201 and themixing mechanism 202. The mixing mechanism is fixedly mounted on theanalyzer. The driving end 301 of the driving device is arranged in acentral hole of the conveying mechanism. In one embodiment, theconveying mechanism 201 is of an internal gear structure; the mixingmechanism 202 is of an external gear structure; the driving end 301 isprovided with a driving rack; the driving rack is engaged with theinternal gear of the conveying mechanism; and the power part 302 is amotor. The reagent kit mixing cavity as shown in FIG. 2 includes amixing reagent bottle 5 with a rotating part 6 at the bottom; thereagent bottle is filled with a magnetic particle reagent to be mixed;and the rotating part 6 is of a gear structure. When the reagent kit 1is placed on the conveying mechanism 201, the gear (the rotating part)mounted at the bottom of the reagent bottle is engaged with the externalgear of the mixing mechanism 202. The motor is powered on to rotate thedriving end, the conveying mechanism is driven by the driving end torotate, and the reagent kit placed on the conveying mechanism is alsorotated together, so that the gear is in transmission with the externalgear of the mixing mechanism. The reagent bottle with the rotating partat the bottom autorotates under the engagement transmission of themixing mechanism and the rotating part, so that the reagent inside thereagent bottle can be uniformly mixed under rotation. In the embodiment,the mixing mechanism is fixedly mounted on the analyzer, the mixingmechanism cannot rotate, and the conveying mechanism is rotated along acentral shaft of the mixing mechanism. In another solution, theconveying mechanism and the mixing mechanism may also rotate relativelyalong the same central shaft.

The transmission mode between the driving end 301 and the conveyingmechanism 201 can also be selected from gear engagement transmission,friction transmission and belt pulley transmission; and the transmissionmode between the mixing mechanism 202 and the rotating part 6 can alsobe selected from gear engagement transmission and friction transmission.

The conveying mechanism and the mixing mechanism are assembled togetherin the form of a bearing structure, so that the friction coefficient ofthe conveying mechanism and the mixing mechanism in the movement processcan be reduced, the mechanical noise of an operating machine can bereduced, the operation energy consumption of the analyzer can bereduced, and the service life of the analyzer can be prolonged. Comparedto the prior art that the conveying mechanism and the mixing mechanismneed to be mounted to the analyzer in separate steps respectively, theconveying mechanism and the mixing mechanism can be mounted as a wholeto the analyzer in the present invention, so that the mounting operationis simple and convenient, and regular repairing, maintenance, cleaningand replacement is facilitated. In addition, it can effectively ensurehorizontal planes of the conveying mechanism and the mixing mechanismare kept in parallel to each other, so that the position relationship ofthe conveying mechanism and the mixing mechanism can be stable, arelatively good dynamic balance state can be maintained, and theoperation precision of the analyzer can be improved. In long-termoperation process, the rotating part and the mixing mechanism can bestably assembled, and the teeth colliding are unlikely to occur. As theconveying mechanism and the mixing mechanism are assembled to form thebearing structure, corresponding fittings on the analyzer can bereduced, so that the mounting space of fittings of the analyzer can besaved. As the driving end is mounted in the central hole of theconveying mechanism, the available space of the analyzer is wellutilized, so that the overall size of the analyzer is relatively small.

In another embodiment, the mixing mechanism 202 is arranged in thecentral hole of the conveying mechanism 201, and the conveying mechanismand the mixing mechanism are assembled together to form the bearingstructure. The conveying mechanism 201 is of an external gear structure,the mixing mechanism 202 is of an internal gear structure, and thedriving end is arranged on the outer side of the conveying mechanism.

In one embodiment, the conveying mechanism includes a base and amounting seat; the base is positioned in the central hole of the mixingmechanism and is connected with the mixing mechanism in the form of abearing structure; the mounting seat is mounted on the base; and themounting seat is used for holding a reagent kit. The mounting seatincludes a transmission part matched with the driving end. The mountingseat is driven by the driving end to rotate; the mounting seat causesthe conveying mechanism to rotate relative to the mixing mechanism; thereagent kit placed on the mounting seat also rotates together, and thusthe rotating part and the mixing mechanism are in transmission. Thereagent bottle with the rotating part at the bottom autorotates underthe transmission of the mixing mechanism and the rotating part, so thatthe reagent inside the reagent bottle is uniformly mixed under rotation.In another embodiment, the conveying mechanism does not include atransmission part matched with the driving end; the mixing mechanismdoes not include a transmission part matched with the rotating part; andthe conveying mechanism and the mixing mechanism are assembled togetherto form a bearing structure. As independent components, the transmissionparts, such as the gear and the friction block, are respectively mountedat corresponding positions of the conveying mechanism and the mixingmechanism.

The reagent cabin 400 of the full-automatic analyzer includes a reagentstoring device and a reagent mixing and conveying device; the reagentmixing and conveying device includes a transport device and a drivingdevice; the transport device includes a conveying mechanism 201 and amixing mechanism 202; the conveying mechanism 201 and the mixingmechanism 202 are sleeved with each other to form a bearing structure;the reagent storing device includes a reagent kit and a reagent kitbracket; the reagent kit bracket 100 is mounted on the conveyingmechanism 201; the reagent kit 1 is put into the reagent kit bracket100; and a rotating part 6 which is connected with a reagent bottlefilled with a reagent to be mixed is matched with the transmission partof the mixing 202. The driving device includes a driving end 301 and apower part 302. When the reagent mixing and conveying device isoperated, the conveying mechanism 201 is driven by the driving device tomove relative to the mixing mechanism, so that the reagent kit isconveyed to a corresponding position for detection. Meanwhile, with therotation of the conveying mechanism, the rotating part for mixing areagent is in transmission with the mixing mechanism, and the reagentbottle autorotates inside the reagent kit under the transmission of themixing mechanism and the rotating part, so that the reagent inside thereagent bottle is uniformly mixed under rotation.

The reagent cabin 400 as shown in FIGS. 18-19 further includes a heatpreservation layer 401 and a cabin cover, so that the reagent cabin isin a relatively heat preserved and closed state; the reagent storingdevice is enclosed by the heat preservation layer, so that the reagentinside the reagent cabin is at a constant temperature; the reagent cabinfurther includes a reagent cabin refrigeration device 403 forrefrigerating the reagent cabin. In one embodiment, the refrigerationdevice is a semiconductor refrigeration piece. In one embodiment, therefrigeration device is arranged at the bottom of the reagent cabin. Thereagent cabin further comprises a reagent bottle zero position sensor404 for determining the initial position of the reagent cabin.

A reagent mixing method of a full-automatic analyzer, which includes thefollowing steps. First, a reagent kit with a test reagent is put into areagent cabin, and the reagent kit is placed on a reagent kit bracket ofthe invention. The reagent kit bracket is matched with the reagentmixing and conveying device of the present invention. When the reagentmixing and conveying device is operated, the driving device of thefull-automatic analyzer drives the conveying mechanism 201 to moverelative to the mixing mechanism, and according to demands of testitems, a corresponding reagent kit is conveyed to reagent acquisitionposition of the analyzer by the conveying mechanism. Meanwhile, with therotation of the conveying mechanism, the rotating part for mixing areagent is in transmission with the mixing mechanism, and the reagentbottles autorotate inside the reagent kit under the transmission of themixing mechanism and the rotating part, so that the reagent inside thereagent bottle is uniformly mixed under rotation.

The invention claimed is:
 1. A reagent mixing and conveying device,comprising a driving device, a transport device and a rotating part,wherein the transport device comprises a conveying mechanism configuredfor conveying a reagent kit and a mixing mechanism for mixing a reagent;wherein the conveying mechanism is configured to be driven by thedriving device to move relative to the mixing mechanism; the rotatingpart and the mixing mechanism are configured to be in transmissionmatching to reduce shaking and to lower noise of the reagent mixing andconveying device; and wherein the conveying mechanism and the mixingmechanism are sleeved with each other to form a bearing structure. 2.The reagent mixing and conveying device according to claim 1, whereinthe conveying mechanism and the mixing mechanism are of annularstructures; and the conveying mechanism is arranged inside a centralhole of the mixing mechanism.
 3. The reagent mixing and conveying deviceaccording to claim 1, wherein the driving device comprises a drivingmotor and a driving end; and the driving end is arranged in a centralhole of the conveying mechanism.
 4. The reagent mixing and conveyingdevice according to claim 3, wherein the transmission mode between thedriving end and the conveying mechanism is selected from gear engagementtransmission, friction transmission and belt pulley transmission; andthe transmission mode between the mixing mechanism and the rotating partis selected from gear engagement transmission and friction transmission.5. The reagent mixing and conveying device according to claim 3, whereinthe conveying mechanism is of an internal gear structure; the mixingmechanism is of an external gear structure; the driving end is providedwith a driving rack; the driving rack is engaged with the internal gearof the conveying mechanism; the rotating part is a gear; the gear as therotating part is engaged with the external gear of the mixing mechanism.6. The reagent mixing and conveying device according to claim 1, whereinthe conveying mechanism comprises a base and a mounting seat; the baseand the mixing mechanism are connected with each other in the form of abearing; the mounting seat is mounted on the base; and the mounting seatcomprises a transmission part matched with the driving device.
 7. A testreagent mixing method, comprising the following steps: (1) providing areagent mixing and conveying device; (2) putting a reagent kit filledwith a reagent to be mixed on the reagent mixing and conveying device;and (3) starting the reagent mixing and conveying device comprises adriving device, a transport part and a rotating part, wherein thetransport device comprises a conveying mechanism for conveying a reagentkit and a mixing mechanism for mixing a reagent; wherein the drivingdevice comprises a driving motor and a driving end and the driving endis arranged in a central hole of the conveying mechanism; wherein theconveying mechanism is driven by the driving device to move relative tothe mixing mechanism; wherein the rotating part and the mixing mechanismare in transmission matching to reduce shaking and lower noise of thereagent mixing and conveying device and the rotating part is used forstirring reagent to be mixed; and the conveying mechanism and the mixingmechanism are sleeved with each other to form a bearing structure. 8.The method according to claim 7, wherein the conveying mechanism and themixing mechanism are of annular structures; and the conveying mechanismis arranged in a central hole of the mixing mechanism.
 9. The methodaccording to claim 7, wherein the conveying mechanism is of an internalgear structure; the mixing mechanism is of an external gear structure;the driving end is provided with a driving rack; the driving rack isengaged with the internal gear of the conveying mechanism; the rotatingpart is a gear; the gear as the rotating part is engaged with theexternal gear of the mixing mechanism.
 10. The method according to claim7, wherein the conveying mechanism is driven by the driving device tomove relative to the mixing mechanism; a corresponding reagent kit isconveyed to a reagent acquisition position of an analyzer by theconveying mechanism; meanwhile, with the rotation of the conveyingmechanism, the rotating part for uniformly mixing the reagent is intransmission with the mixing mechanism; a reagent bottle autorotatesinside the reagent kit under the transmission of the mixing mechanismand the rotating part, so that the reagent in the reagent bottle isuniformly mixed under rotation.
 11. The method according to claim 8,wherein the conveying mechanism is driven by the driving device to moverelative to the mixing mechanism; a corresponding reagent kit isconveyed to a reagent acquisition position of an analyzer by theconveying mechanism; meanwhile, with the rotation of the conveyingmechanism, the rotating part for uniformly mixing the reagent is intransmission with the mixing mechanism; a reagent bottle autorotatesinside the reagent kit under the transmission of the mixing mechanismand the rotating part, so that the reagent in the reagent bottle isuniformly mixed under rotation.
 12. The method according to claim 9,wherein the conveying mechanism is driven by the driving device to moverelative to the mixing mechanism; a corresponding reagent kit isconveyed to a reagent acquisition position of an analyzer by theconveying mechanism; meanwhile, with the rotation of the conveyingmechanism, the rotating part for uniformly mixing the reagent is intransmission with the mixing mechanism; a reagent bottle autorotatesinside the reagent kit under the transmission of the mixing mechanismand the rotating part, so that the reagent in the reagent bottle isuniformly mixed under rotation.